Optimizing Preclinical PV Loop Techniques and Analysis in Mice

Optimizing Preclinical PV Loop Techniques and Analysis in Mice

  • 29 Apr 22

这是有用的网络研讨会，对于有兴趣开始压力销量获取和分析的临床前研究人员，或者那些已经使用PV循环的人可能会受益于在整个过程中增加和实验能力的提示。

Abstract:

在本次会议上，贝克学院的临床前心脏病学显微外科手术和成像平台的Daniel Donner博士和Aascha Brown博士将使用合并的Adinsmtruments和Millar System为小鼠的压力量导管插入术提供最佳实践建议。通过设备清单，连接和设置将提供完整的演练；通过介绍PV的LabChart工作流程以及如何优化从获取PV循环到如何分析它们的工作流程。

The Baker Insititute's Preclinical Cardiology Microsurgery and Imaging Platform specialize in myocardial ischemia-reperfusion models, novel cardiac arrest, and aortic constriction models for preclinical trials. This webinar includes a step-by-step surgical procedure using the carotid N-S approach (with short accompanying video) using a Millar SPR-839 1.4F with a mouse model.

Key Topics:

  • Recommended equipment list:For surgical procedures in mouse model
  • 使用PV循环的重要性：A gold standard approach in hemodynamic studies
  • Introduction to PV assessments:Including compliance and inotropy / interpreting the loop
  • 外科手术设置：Including preparation tips, equipment setup and connection, overview of the MPVS interface, channel setup, introduction to the PV loop and workflow setup in LabChart 8.
  • Surgical Procedure Walkthrough:Including overview of procedural steps, carotid isolation and cannulation, supporting surgical video footage
  • Post Surgery Calibrations:Walkthrough of cuvette, saline, baseline and alpha calibration with tips for efficiency to support throughput.
  • Overview of occlusions analysis
  • Further calibration advice:SV/CO校准对电导测量的重要性，其中数据差异的示例

相关产品：

Millar Mikro-TipTM值鼠标PV导管»
Millar MPVS Ultra Single Segments PV Unit»
Millar MPVS Ultra电缆包»
ldsports体育资讯
研究PowerLabs»
生物放大器»
T-Type Pod»
热电偶探针»

Additional resources:

Read
为什么您应该在研究中加入压力卷循环»
光伏循环简介：理解光伏循环的点和心脏功能的度量»
PV技巧和技巧：用于测量老鼠压力卷循环的专家建议»
Importance of PV Catheter Calibration in Admittance and Conductance Systems »

Watch
Three Part Mini Webinars: Industry Based Experts on Preclinical Cardiovascular Research »
Webinar: Understanding Ventricular Pressure-Volume Catheter Calibrations and Experimental Design »
Webinar: Cardiac PV Loop Data Analysis Tips and Tricks »
Surgical Instruction Video Series: Pressure Volume (PV) and Invasive Pressure »

About the speakers:

丹尼尔·唐纳（Daniel Donner Donner）博士PV网络研讨会ADI

Dr Daniel Donner - PhD, CertLead, GCertResMgt, MMEdRes, BBiomedSc
研究官
贝克心脏和糖尿病研究所
丹尼尔（Daniel）是临床前心脏病学显微外科手术和成像平台的负责人，以及AD仪器压力卷系统的微型外科教练。国际工业和学术合作伙伴参与了他的团队在临床前心脏病学模型和表型方面的专业知识，以进行临床前试验策略，设计和交付

Dr Aascha Brown Mouse PV webinar ADI

Dr Aascha Brown - PhD, BBiotech (Hons)
显微外科和成像技术官
贝克心脏和糖尿病研究所
Aascha是该平台的主要显微外科手术和成像专家。布朗博士通常为其许多合作伙伴分析大量P-V数据，并为优化工作流提供宝贵的见解。

PV loop pressure volume analysis software LabChart ADI

LabChart- PV Loop 2.5 for left and right ventricle studies.

ThePV循环分析软件模块用于LABCHARTis specifically designed for the analysis of体内小动物和大型动物的心室压力体积数据，或离体, using working heart systems. Analyze ventricular pressure-volume baseline and occlusion data in realtime or post acquisition.
接触us了解更多信息。