Three benefits of isolated organ research

What is isolated organ research - and why should you be doing it?

As the name implies,isolated organ researchinvolves the removal of an organ from the body and maintaining its physiological function for experimental purposes.

因此，对于那些尚未亲自尝试这项技术的人来说，这是孤立的器官研究可能在您的研究实验室受益的三个原因！

孤立器官研究的三个关键好处

1.清晰度和速度

Normally, when physiological conditions change, the body adapts accordingly to maintain systemic homeostasis, which can result in many physiological parameters changing simultaneously. By removing the organ and studying it in a controlled environment, recorded responses are free from bodily influences,which can be unseen or unknown。This means when it comes to the analysis, you can gain clearer and more definitive results in a relatively easy and quick manner.

此外，您还具有能够记录实验过程中可能发生的颜色或形态变化等视觉观察的额外优势，从而提供了其他定性数据。

2.完整的环境控制

Control of the isolated organ environment is made easy using accurate and precise monitoring and recording tools, such as our温度和pH套件，o₂＆co₂成套工具，，，，andTransonic flowprobes and flowsensorswhich can be added onto any of our孤立的器官系统。

3. Flexibility and direct measurements

Lastly, with an isolated organ system, you have the flexibility to easily scale up or down the complexity of the experiment protocol as required by the study. In addition, since it is an invasive method, you can directly place sensors or electrodes on the organ to increase measurement precision and accuracy.

相关：如何建立典型的孤立灌注啮齿动物肝（IRPL）实验。

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ADInstruments Advantage

ADInstruments presents you with the opportunity to measure and analyze a variety of different signals from an isolated organ preparation using the same recording and analysis platform. Using ourPowerLabdata acquisition system andLabChart分析软件，您可以同时在同一软件中记录不同的信号，并在记录的信号之间易于辨别关系。

例如，如果您想查看特定药物对孤立心脏的药理作用 - 您可以对心脏进行不同浓度的药物，同时测量心脏参数等心脏参数的任何变化（（ECG），收缩力（使用bridge ampand力传感器）orblood pressure。然后使用LabChart的Dose-Response Module，您可以快速自动化相应剂量反应曲线的推导，自动计算EC₅₀价值。

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想开始我们孤立的肝系统，但不知道从哪里开始？Check out our short guide on key considerations when performing an IPRL experiment.

有关的:

Effects of constant flow vs. constant pressure perfusion on Langendorff isolated heart studies

Langendorff与工作心脏灌注 - 有什么区别？

Choosing the right perfusate for a Langendorff Isolated heart preparation

Watch webinar: An introduction to isolated Langendorff heart – Experimental considerations and best practices

帮助（孤立）心脏的问题：心血管和药理学研究的简单性和灵活性

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References

Yeh T.，Wechsler A.S.（1998）研究中的孤立器官。在：Trodl H.，McKneally M.F.，Mulder D.S.，Wechsler A.S.，McPeek B.，Spitzer W.O.（eds）手术研究。斯普林格，纽约，纽约

Shatoor, Prof. Abdullah & Al-Hashem, Professor Fahaid & Elkarib, Abbas & Sakr, Hussein & Alkhateeb, Mahmoud. (2012). EC50 of adrenaline-atenolol: Functional agonist assay using Langendorff isolated rabbit heart tethered to PowerLab data acquisition system. African journal of pharmacy and pharmacology. 6. 1092-1098. 10.5897/AJPP12.123.

Bessems M等。2006年，孤立的灌注大鼠肝脏：实验动物的定期模型的标准化。